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Development of analytical methods for the assessment of water and nutrient uptake

Work package 4 was used in Phase I to adapt and develop analytical methods to identify and quantify water and nutrient uptake from the subsoil. For this purpose, the 18O signature of water (δ18Ow) at different soil depths and xylem sap in the plant, the 18O signature in soil and xylem phosphates (δ18OP) as a tracer for P uptake, and the δ15N signature as an indicator for nitrogen uptake were tested. As proxies for cationic macro- and micronutrients as well as anionic micronutrients, methods were developed for the analysis of the δ26Mg and δ56Fe signatures.

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